Interestingly, plant leaves are covered by a cuticle consisting of cutin, an esterified aliphatic polymer that is impregnated and overlaid by intra- and epi-cuticular waxes consisting of very long-chain aliphatic compounds, including alkanes, alcohols, and fatty acids. The described strain was isolated from plant leaf material. Pseudomonas citronellolis has long been recognized for its ability to degrade hydrocarbons such as isoprenoid compounds including citronellol, hence its name, and complex oily sludge contaminations, indicating the species might potentially serve as bioremediation organism by cleaning up oil contaminated sites. Thus, a comprehensive picture of genetic diversity within the pseudomonads is lacking and investigating thus far unsequenced species will fill this knowledge gap. Taking into account the assigned species of the sequenced strains only, roughly a third (76 of 199) of the validly published Pseudomonas species have been sequenced. aeruginosa strains and 105 plant pathogenic P. Up till now, this database is heavily biased towards pathogenic species, most prominently including 996 human-pathogenic P. at the time of writing the Pseudomonas Genome Database contained 98 complete genomes and 1447 draft genomes.
#Microsynth lattuce full#
Due to their versatile properties and pathogenicity, pseudomonads garnered much attention in recent years and members of the genus have been the subject of full genome sequencing projects, i.e. The genus Pseudomonas encompasses a large group of bacteria that are ecologically and functionally very diverse including many human and plant pathogenic species, but also species with mutualistic host interactions, the most prominent examples of which are biocontrol strains that protect plants from pathogens. The genome is discussed with respect to a phyllosphere related lifestyle, aliphate and terpenoid degradation, and antibiotic resistance. citronellolis type strain PRJDB205 not yet publicly available. The availability of the genome will be helpful for comparative genomics of the phylogenetically broad pseudomonads, in particular with the sequence of the P.
To evaluate the potential of this strain as source of transferable antibiotic resistance genes on raw consumed herbs we therefore investigated if those resistances are encoded on mobile genetic elements. Furthermore, the isolated strain exhibited resistance to several antibiotics. In addition, basil leaves are known to contain high amounts of terpenoid substances, hinting to a potential nutrient niche that might be exploited by P. Plant leaves are covered by long-chain aliphates making up a waxy layer that is associated with the leaf cuticle. citronellolis has been the subject of many studies including the investigation of long-chain aliphatic compounds and terpene degradation. The 6.95 Mbp genome contains 6071 predicted protein coding sequences and 96 RNA coding sequences. Here we describe the physiology of this microorganism, its full genome sequence, and detailed annotation. We isolated strain P3B5 from the phyllosphere of basil plants ( Ocimum basilicum L.). Pseudomonas citronellolis is a Gram negative, motile gammaproteobacterium belonging to the order Pseudomonadales and the family Pseudomonadaceae.